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17 kinds of operation tips of elisa kit, how much do you know?
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There are many kinds of ELISA kits in China, such as ELISA test kit, ELISA kit, enzyme-linked immunosorbent kit, enzyme-linked immunosorbent assay kit, enzyme-linked immunosorbent assay kit, enzyme-free kit, etc. The name is
ELISA test kit, enzyme-linked immunosorbent assay kit, and the like.
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The ELISA kit is suitable for qualitative detection of anti-human hepatitis E (HEV) virus IgM antibody ELISA in human serum or plasma.
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Since its inception in the 1960s and 1970s, ELISA kits have been recognized and respected by researchers all over the world, and have been widely promoted in Europe, America, and China, especially in the domestic biochemical field.
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The Elisa biological test is a highly sensitive, specific, and reproducible experimental diagnostic method. Because of its stable reagents, easy storage, easy operation, and objective judgment, it has been widely used in various fields of immunology.
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So what operational skills does it have?
Today, Shanghai Weiwei Biotechnology Co., Ltd. Chen Technology tells you about 17 kinds of operation skills of ELISA kit. If you master these experimental techniques, you will be more proficient in the ELISA experiment and greatly reduce the experiment. The risk, the newcomer to the ELISA kit experiment, after mastering this knowledge, will operate much faster.
The 17 related operation skills summarized by the ELISA kit Chen technology are as follows:
1. Remember to use a blotting paper to gently blot and dry on the surface of the plate after adding the enzyme reagent.
2. Arrange the amount of detection reasonably so as to avoid long waiting time for washing the plate.
3. When sucking liquid, it is necessary to remove the gun with a dose and a quantity close to the required amount to reduce the error.
4. Be sure to do a two-hole experiment so that the accuracy of the data can be guaranteed and the precision of the kit can be reflected.
5. The sample diluent is filled with a dosing device and often corrected for accuracy.
6. In order to prevent the sample from evaporating, the reaction plate is placed in a closed box covered with a damp cloth, and the plate is covered with a cover or a film.
7. Unused plate or reagent, please store at 2-8 °C. Horseradish peroxidase-labeled anti-human IgG working solution should be used according to the required amount. Do not reuse the diluted horseradish peroxidase-labeled anti-human IgG working solution.
8. In the ELISA kit experiment, the incubator should be placed in time after the sample is added. When there are more specimens, it must be operated in batches. According to the instructions, the operation time is strictly controlled to prevent the incubation time from being artificially extended, resulting in non-specific binding surrounding the reaction well, which is difficult to clean thoroughly.
9. The remaining samples and waste should be sterilized by autoclaving at 121 °C for 30 minutes, or treated with a disinfectant such as 5.0 g/L sodium hypochlorite for 30 minutes.
10. When the human ELISA kit is washed, each well needs to be filled with liquid to prevent the free enzyme in the orifice from being washed.
11. One hole was left for each experiment as a blanking zero hole. No additional reagent was added to the well, except that the substrate solution and 2N H2SO4 were added. Use this hole to adjust the OD value to zero when measuring.
12. After washing the plate manually, add the washing solution each time. Should be allowed to stand for 15 ~ 30s, do not splash the washing liquid in one enzyme label L into another enzyme label hole to prevent cross-contamination. After removing the washing solution, place the microplate on a towel or absorbent paper and pat dry.
13. When there is doubt about the results of the sample, other test methods are required for verification.
14. When there is no deionized water or double distilled water, you can use Wahaha pure water to prepare the solution. Do not use tap water.
15. When using the micro-sampler, replace the tip with the liquid in different bottles, even if the standard solution is taken.
16. When the liquid is sucked, the speed is not too fast, so as to avoid air bubbles, and the amount of the human ELISA kit is not accurate enough.
17. When sucking the liquid, use a micro-sampler with a close range and a required amount to reduce the error.
Shanghai Jiwei Biotechnology Co., Ltd. is a professional manufacturer of various types
High-tech biotechnology company with ELISA kit, the company has a wide range of ELISA kits, complete species, covering species: human ELISA kit, mouse ELISA kit, rat ELISA kit, hamster ELISA kit, guinea pig ELISA Kit, pig ELISA kit, bovine ELISA kit, sheep ELISA kit, canine ELISA kit, plant ELISA kit, etc., the company adheres to the quality of the fundamental, for the majority of scientific research scholars to provide better use of ELISA kits!
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