Goat ELISA kit reveals no worries about diabetes - Huaqiang

The elisa kit reveals a dual-cause mechanism for the determination of human thrombin levels in specimens using a double antibody sandwich assay. The microplate is coated with purified human thrombin antibody to prepare a solid phase antibody, and thrombin is sequentially added to the microcapsule of the coated monoclonal antibody, and then combined with the HRP-labeled thrombin antibody to form an antibody-antigen-antibody standard antibody. The complex, after thorough washing, was added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with thrombin in the sample. The absorbance was measured at 450 nm using a microplate reader, and the human thrombin concentration in the sample was calculated from a standard curve.

1. Highly efficient, sensitive and specific antibodies;

2. Stable repeatability and reliability;

3. A solid phase carrier with good adsorption performance, low blank value and high transparency at the bottom of the hole;

4. Applicable to a variety of specimen types such as serum, plasma, tissue homogenate, cell culture supernatant, urine, etc.;

5. Saving experimental expenses.

The ELISA kit says that these drugs have been widely used to effectively control the characteristic symptoms of type 2 diabetes - hyperglycemia, but they only deal with the consequences of diabetes, and do not solve its underlying cause, so it can only be controlled and cannot be cured. disease. ”

New research suggests that normal glycemic regulation depends on the partnership between pancreatic insulin-producing cells and the hypothalamic and other neuronal circuits in the brain region that are closely involved in maintaining normal blood glucose levels. The authors believe that failure of both the islet cell system and this brain-centered glycemic regulation system is essential for the development of type 2 diabetes.