How to determine the results of ELISA kit experiments - Database & Sql Blog Articles

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How to determine the results of the elisa kit experiment

When we are ready for the experiment and are about to finish, how do we determine the results of the ELISA kit http://? They are quantitative, semi-quantitative, and qualitative. Let's take a look.
1. Quantitative determination: ELISA is performed after a series of dilutions using a known standard. The sample is simultaneously measured with the sample to be tested, and a standard curve is drawn. The result is expressed in absolute or active units.
2. Semi-quantitative determination: The results are generally expressed in titers. After serial dilution of the specimen, an ELISA test is performed, and the highest dilution above the positive cutoff value is the "titer" of the specimen.
3, qualitative determination (1) positive determination value method (cut-off value, CO value): generally the negative control average A value plus a constant, reagent preparation is strictly standardized, first calculate the positive control A value average and negative The average of the control A values. Specimen A value ≥ CO value is positive.

(2) Inhibition rate method: In the competition inhibition method, the result can be expressed by the inhibition rate. Inhibition rate (%) = (A negative control A-A to be tested) / negative control X 100%, generally positive inhibition rate ≥ 50%.

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